Download the fantastic book titled RT PCR Protocols written by Nicola King, available in its entirety in both PDF and EPUB formats for online reading. This page includes a concise summary, a preview of the book cover, and detailed information about "RT PCR Protocols", which was released on 04 February 2008. We suggest perusing the summary before initiating your download. This book is a top selection for enthusiasts of the Science genre.
Summary of RT PCR Protocols by Nicola King PDF
Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.
Detail About RT PCR Protocols PDF
- Author : Nicola King
- Publisher : Springer Science & Business Media
- Genre : Science
- Total Pages : 370 pages
- ISBN : 159259283X
- Release Date : 04 February 2008
- PDF File Size : 41,5 Mb
- Language : English
- Rating : 4/5 from 21 reviews
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